detection of cytolethal distending toxin (cdt) genes of campylobacter jejuni and coli in fecal samples of pet birds in iran

نویسندگان

fateme ehsannejad

arzhang sheikholmolooki

mohammad hassanzadeh

roja shojaei kavan

mohammad soltani

چکیده

background: campylobacteriosis is one of the most commonbacterial intestinal disorders of humans in many countries.a wide range of domestic and wild bird species have been identifiedas natural reservoirs. objectives: the aim of this studywas to determine the campylobacter spp from pet birds andscreen the determined campylobacters for presence of virulencecytolethal distending toxin (cdt) genes. methods: a totalof 660 fecal samples from 32 different species of pet birds weretaken and examined for detection of campylobacter spp. andwere investigated for presence of cdt genes. all the sampleswere collected from clinically healthy birds that were kept incage, zoological parks, and/ or in zoo of tehran, the capital ofiran. results: in total, 20 thermophilic campylobacter weredetected from 8 different avian species. from 20 confirmedcampylobacter spp., 16 samples (80%) were c. jejuni positiveand 4 (20%) were c. coli in species-specific pcr test. furthermore,out of 20 detected campylobacter, 13 (65%) harboredthe various subunits of cdta, cdtb and cdtc genes, and 7 (35%)were negative for all tested cdt genes. conclusions: our findingsindicate that the carriage rate of campylobacter in differentspecies of cage and/or in zoo birds is high and confirm thatcdt genes may frequently be present in campylobacter spp.

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منابع مشابه

Detection of cytolethal distending toxin (cdt) genes of Campylobacter Jejuni and Coli in fecal samples of pet birds in Iran

BACKGROUND: Campylobacteriosis is one of the most commonbacterial intestinal disorders of humans in many countries.A wide range of domestic and wild bird species have been identifiedas natural reservoirs. OBJECTIVES: The aim of this studywas to determine the Campylobacter spp from pet birds andscreen the determined Campylobacters for presence of virulencecytolethal distending toxin (cdt) genes....

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Detection of cytolethal distending toxin (cdt) Genes in Campylobacter jejuni and Campylobacter coli isolated from the intestinal of commercial broiler chickens, turkey and quail of Iran

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Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: detection and analysis by PCR.

Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter coli strains tested. Restriction analyses of the cdt PCR products showed clear differences betwee...

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detection of cytolethal distending toxin (cdt) genes in campylobacter jejuni and campylobacter coli isolated from the intestinal of commercial broiler chickens, turkey and quail of iran

background: campylobacter jejuni and campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. sharing of the cytolethal distending toxin (cdt) genes in campylobacter is common and is considered species specific. objectives: in this study we focused on detecting the presence of cdt gene in c. jejuni and c. coli isolated from broilers, turkeys and quails of ir...

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Investigation of Virulence-Associated genes and Cytolethal Distending Toxin Production in Campylobacter spp. isolated from broilers

The aim of this study was to investigate the prevalence of virulence and Cytolethal Distending Toxin (CDT) genes in the Campylobacter isolates from intestinal contents and gall bladders of broilers and, to evalute their cytotoxic effects on HeLa cell cultures. These genes play important roles in bacterial adherence to intestinal mucosa, flagella-mediated motility, invasive capability and the ab...

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عنوان ژورنال:
iranian journal of veterinary medicine

ناشر: دانشگاه تهران

ISSN 2251-8894

دوره 9

شماره 1 2015

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